DNA Polymerases
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Filtered Search Results
Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment
DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | DNA Polymerase I |
|---|---|
| Content And Storage | DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C. |
| Hot Start | No |
Invitrogen™ T4 DNA Polymerase
DNA polymerase with 3'-exodeoxyribonuclease activity, but not 5'a3' exodeoxyribonuclease activity
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Polymerase | T4 DNA Polymerase |
| Content And Storage | T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C. |
| Description | DNA Polymerase |
| Hot Start | No |
| Exonuclease Activity | 3' - 5' |
Thermo Scientific™ T7 DNA Polymerase (10 U/μL)
Catalyze DNA synthesis in the 5'≥3' direction with T7 DNA Polymerase, a template dependent DNA polymerase.
MP Biomedicals™ RapidTaq PCR MasterMix (2X)
RapidTaq PCR MasterMix (2X) provides rapid PCR reactions and is ready for direct loading on agarose gels.
Promega GoTaq™ G2 Colorless Master Mix
GoTaq(R) G2 Master Mixes are premixed ready-to-use solutions containing GoTaq(R) G2 DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of a wide range of DNA templates by PCR.
| Content And Storage | −30° to −10°C |
|---|---|
| Description | Colorless master mix |
| For Use With (Application) | RT-PCR |
Promega GoTaq™ G2 Flexi DNA Polymerase
GoTaq™ G2 Flexi DNA Polymerase is supplied with 5X Green GoTaq™ Flexi Buffer and 5X Colorless GoTaq™ Flexi Buffer and 25mM MgCl2.
| Content And Storage | −30° to −10°C |
|---|---|
| Description | Flexi DNA Polymerase |
| Concentration | 5 U/μL |
| For Use With (Application) | RT-PCR |
Promega GoScript™ Reverse Transcriptase
GoScript Reverse Transcriptase utilizes M-MLV and state-of-the-art buffer technology designed for qPCR to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors.
| Includes | GoScript reverse transcriptase, GoScript 5X reaction buffer, MgCl2 |
|---|---|
| Content And Storage | −30 to −10°C |
| Description | GoScript Reverse Transcriptase |
| Form | Liquid |
| pH | 7 |
| For Use With (Application) | cDNA Synthesis,RT-PCR |
Promega GoTaq™ G2 DNA Polymerase
GoTaq™ G2 DNA Polymerase is supplied with 5X Green GoTaq™ Reaction Buffer and 5X Colorless GoTaq™ Reaction Buffer.
| Content And Storage | −30° to −10°C |
|---|---|
| Description | DNA Polymerase |
| Concentration | 5 U/μL |
| For Use With (Application) | RT-PCR |
Promega GoTaq™ Flexi DNA Polymerase
Taq DNA Polymerase. Provided with Green and Colorless Reaction Buffers without MgCl2, and 25mM MgCl2. Green Buffer allows direct gel analysis after amplification. Enhanced performance over standard Taq DNA Polymerase.
| Includes | GoTaq Flexi DNA Polymerase, 5X Colorless GoTaq Flexi buffer (Used when direct fluorescence or absorbance readings are required without prior purification of the DNA from the amplification reaction), 5X Green GoTaq Flexi buffer, 25mM MgCl2 |
|---|---|
| Content And Storage | −30 to −10°C |
| Description | DNA Polymerase |
| Form | Liquid |
| pH | 8 |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
Promega M-MLV Reverse Transcriptase
M-MLV RT is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb).
| Content And Storage | −30 to −10°C |
|---|---|
| Storage Buffer | Tris-HCl (pH 7.5 at 25°C) 20mM, NaCl 0.2M, EDTA 0.1mM, DTT 1mM, 0.01% Nonidet™ P40™ and Glycerol 50% |
| Form | Liquid |
| For Use With (Application) | cDNA Synthesis |
| Source | Recombinant E. coli Strain |
Promega GoTaq MDx DNA Polymerase, 100u
GoTaq MDx DNA Polymerase is a full-length form of Taq DNA polymerase that exhibits 5'→3' exonuclease activity.
Promega GoTaq MDx DNA Polymerase, Glycerol-Free, 500u
GoTaq MDx DNA Polymerase is a full-length form of Taq DNA polymerase that exhibits 5'→3' exonuclease activity.
Promega GoTaq MDx Hot Start Polymerase, High Concentration, 1,000u
GoTaq MDx Hot Start Polymerase contains GoTaq MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity.
Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant
Point mutation of M-MLV RT resulting in no RNase H activity. Suitable for rare or long transcripts.
| Content And Storage | −30 to −10°C |
|---|---|
| Description | M-MLV Reverse Transcriptase |
| Storage Buffer | Tris-HCl (pH 7.5 at 25°C) 20mM, NaCl 200mM, EDTA 0.1mM, DTT 1mM, 0.01% Nonidet™ P40™ and Glycerol 50% |
| Form | Liquid |
| For Use With (Application) | cDNA Synthesis |
| Source | Recombinant E. coli Strain |
Promega AMV Reverse Transcriptase
AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.
| Content And Storage | −30 to −10°C |
|---|---|
| Storage Buffer | Potassium Phosphate (pH 7.2 at °C) 200mM, 0.2% Triton™ X-100, DTT 2mM and Glycerol 50% |
| Form | Liquid |
| For Use With (Application) | cDNA Synthesis |
| Source | Avian Myeloblastosis Virus |